Geranylgeraniol Supplementation Mitigates Muscle Atrophy With Mitochondrial Quality Improvement In Diabetic Rats

With diabetes, skeletal muscle mitochondrial quality control (mitochondrial fusion, fission & macro-autophagy) is impaired. Geranylgeraniol (GG) shown to have a protective effect on preventing damage and health; however, the of GG diabetic model not known. PURPOSE: To determine cross-sectional area (CSA) in rats. METHODS: Thirty-five Sprague-Dawley rats were divided into three diet groups: (CON), high-fat with 35 mg/kg body weight streptozotocin (HFD), HFD 800 (GG). Due limited sample, total 21 (CON: n = 7; HFD: GG: 7) rats’ samples used for this report. The soleus muscles harvested after 7-weeks feeding analyzed OPA1, MFN2, DRP1, pDRP, PINK1, Parkin, LC3A, LC3B protein content using western blot analysis. Muscle CSAs assessed Image J. RESULTS: A significant (p < 0.05) condition was observed contents CSA. For (0.21 ± 0.08) had lower MFN2 than CON (0.43 0.04; p 0.007) (0.65 0.08; 0.010). fission, (0.26 0.07) DRP1 (0.59 0.07; 0.019). macro-autophagy, (1.08 0.28) LC3A (2.81 0.55; 0.028) (3.99 0.57; 0.010); whereas (0.63 0.21) (1.93 0.24; 0.012). No differences LC3B/A. size, (10,092.88 104.67μm2) larger CSA (7284.69 70.91μm2, 0.001) (5615.59 59.97μm2; 0.001), 70.91μm2) 0.001). CONCLUSION: supplementation could prevent fragmentation (reduction DRP1), thus, potentially resulting decreased demand fusion MFN2). In addition, greater rate autophagosome degradation formation LC3B) (indicative an increase macro-autophagy). Improvement contribute attenuating reduction size supplementation.